Search results for "Lymphoproliferative response"

showing 3 items of 3 documents

Assessment of human cytomegalovirus specific T cell immunity in human immunodeficiency virus infected patients in different disease stages following …

2004

T cell immunity to human cytomegalovirus (HCMV) was assessed in HAART-treated HIV-1 infected patients (9 asymptomatic, CDC group A; and 22 symptomatic, CDC group B), and in eight HIV-1 long term non-progressors. Patients were either prospectively or cross-sectionally examined for CD4(+) T cell counts, HIV RNA load, HCMV leukoDNAemia, HCMV DNA in urine, lymphoproliferative response (LPR) to HCMV and phytohemagglutinin (PHA), and cytokine secretion (IFN-gamma and IL-4) by HCMV-stimulated peripheral blood mononuclear cell (PBMC) cultures. No patient either progressed to clinical AIDS or developed HCMV active infection during the study period. Twenty-nine patients responded to HAART, though 12 …

Human cytomegalovirusAdultMaleAdolescentvirusesT cellT-LymphocytesCongenital cytomegalovirus infectionCytomegalovirusViremiaHIV InfectionsBiologyIn Vitro TechniquesLymphocyte ActivationPeripheral blood mononuclear cellAsymptomaticHIV Long-Term SurvivorsVirologyAntiretroviral Therapy Highly ActivemedicineHumansvirus diseasesbiochemical phenomena metabolism and nutritionMiddle Agedmedicine.diseaseVirologyInfectious Diseasesmedicine.anatomical_structureImmunologyCytokinesCytokine secretionFemalemedicine.symptomLymphoproliferative responseJournal of medical virology
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Human cytomegalovirus (HCMV)-specific CD4+ T lymphocyte response in AIDS patients with no past or current HCMV disease following HAART.

2003

Abstract Background: The incidence of Human Cytomegalovirus (HCMV) end-organ disease has dramatically decreased since the implementation of highly active antiretroviral therapies (HAARTs), but the precise immune mechanism whereby HCMV is controlled remains to be elucidated. Objectives: To investigate the effect of (HAART) on CD4 + T-cell immunity to HCMV in AIDS patients with no past or current HCMV disease. Study design: Seventeen patients were prospectively examined for CD4 + (CD45RO + and CD45 RA + ) T-cell counts (flow cytometry), HIV RNA load (Amplicor HIV test), HCMV leukoDNAemia and HCMV DNA in urine (nested PCR), lymphoproliferative response (LPR) to HCMV, phytohemagglutinin (PHA) a…

Human cytomegalovirusAdultCD4-Positive T-LymphocytesMalevirusesCytomegalovirusmedicine.disease_causeLymphocyte ActivationHerpesviridaeVirusInterferon-gammaBetaherpesvirinaeT-Lymphocyte SubsetsVirologyImmunopathologyAntiretroviral Therapy Highly ActivemedicineHumansViremiaAcquired Immunodeficiency SyndromebiologyAIDS-Related Opportunistic Infectionsvirus diseasesHIVbiochemical phenomena metabolism and nutritionMiddle AgedViral Loadbiology.organism_classificationmedicine.diseaseVirologyCD4 Lymphocyte CountInterleukin-10Infectious DiseasesImmunologyCytomegalovirus InfectionsDNA ViralCytokinesRNA ViralCytokine secretionFemaleViral diseaseInterleukin-4Lymphoproliferative responseJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology
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Application of a 5-bromo-2′-deoxyuridine ELISA for measuring the lymphoproliferative response to human cytomegalovirus in HIV-1-infected patients

2002

Assessment of the lymphoproliferative response to human cytomegalovirus (HCMV) may help to identify human immunodeficiency virus (HIV)-1-infected patients at high risk of developing HCMV end-organ disease. The tritiated thymidine ([3H]-TdR)-incorporation assay is the gold standard for measuring lymphoproliferative responses, though it is unsuitable as a routine laboratory procedure. An alternative non-radioactive technique, a 5-bromo-2'-deoxyuridine (BrdU) enzyme-linked immunosorbent assay, was applied for measuring T-cell proliferation in response to HCMV. Stimulation of either 1 x 10(5) or 5 x 10(4) peripheral blood mononuclear cells (PBMCs)/well with 10 PFU/well (before inactivation) of …

Human cytomegalovirusCellular immunityvirusesCytomegalovirusEnzyme-Linked Immunosorbent AssayBiologyLymphocyte ActivationPeripheral blood mononuclear cellViruschemistry.chemical_compoundVirologymedicineHumansAcquired Immunodeficiency SyndromeAIDS-Related Opportunistic Infectionsvirus diseasesmedicine.diseaseVirologyDeoxyuridineBromodeoxyuridinechemistryCytomegalovirus InfectionsHIV-1Indicators and ReagentsThymidineLymphoproliferative responseBromodeoxyuridineJournal of Virological Methods
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